The aim of this study was to compare the acute physiological responses to five bout of high intensity interval exercise within and between pyramid interval training group (PITG) and basic interval training group (BITG) in university male handball players. Twelve (12) male handball players were selected at random as subjects, who volunteered to participate in this study. The result of the study showed that interaction effect of maximum heart rate (HRmax) displayed significance, F
= 5.58, p
= 0.203 - large effect but average heart rate (HRave) showed no significance, F
= 0.531, p
= 0.024 - small effect. Further, result showed that Pyramid interval training (PIT) found higher than basic interval training (BIT) and both PIT and BIT showed significant difference within the group. It is concluded that PIT had comparative physiological impact like BIT and increasingly valuabl e for the coaches or strength and conditioning coaches to adopt pyramid interval training.