The purpose of the study was to evaluate the effectiveness of plyometric training in developing cardiovascular endurance of Kho-Kho players. For this purpose, thirty regular practicing female Kho-Kho players (age 19.41 ± 1.32 years; height 160.41 ± 3.53 cm and weight 53.18 ± 3.23 kg) randomly divided into two groups- Experimental and Control. Six weeks plyometric training with 2-day sessions per week was employed on Experimental group along with their regular Kho-Kho practice. Control group took part only in their regular Kho-Kho practice. Cardiovascular endurance was measured by 600 m Run-walk test prior to and after 6 weeks intervention period. Collected data were analyzed through Pair t-Test with level of significance fixed at 0.05 level. According to the result, improvement of cardiovascular endurance of experimental group was statistically significant; where as no significant improvement was observed in Control group.