The purpose of this study was to compare the selected physical and physiological variables among university men volleyball, basketball and handball players. To achieve this purpose of the study, twenty men volleyball players, twenty basketball players and twenty handball players from Colleges affiliated to Tamil Nadu Physical Education and sports University, Chennai, Tamil Nadu, India were selected as subjects. The data were collected for all subjects on selected physical fitness qualities such as speed, agility and cardio respiratory endurance by using 50mts run, shuttle run and cooper’s 12 min run / walk test. The one way analysis of variance was used to find out the significant difference among university men volleyball, basketball and handball players. The Scheffe’s test was used as a post hoc test to find out the paired mean differences, if any. In all cases, .05 level of confidence was fixed to test the significance, which was considered as an appropriate. The results of the showed that there was a significant difference among university men volleyball, basketball and handball players on speed, agility and cardio respiratory endurance.