Present study was conducted to assess and evaluate the differential effects of yoga and physical workout on psycho-physiological variables among students of senior secondary school appearing for board exams. For the purpose of the study total 60 number of students were selected and they were divided in two equal groups i.e. Group I (30 students) and Group II (30 students). Group 1 subjects were made to perform yoga practices and Group 2 subjects were made to perform physical workout of forty minute daily for twelve weeks. Physiological variables like heart rate, respiratory rate, and blood pressure were measured. Maudsley Personality Inventory (MPI) was used to evaluate the neuroticism and extroversion traits of the school students. Inventories developed by Defense Institute for Physiology & Applied Sciences (DIPAS) have been used to assess traits of anger and kind of persona. In the study, it was observed that there was no significant rise in pulse rate of Group 1 students, and increase in pulse rate in Group 2 at stressor 1 but become non-significant at stressor 2 in comparison to their baseline level. No change in neuroticism scores was observed in Group 1 students but there was a significant increase in Group 2. Increase in extraversion scores was found in Group 1 students both at stressor 1 and stressor 2 whereas, significant increase in extraversion scores in Group 2 students was seen only at stressor 2. Significant decrease in anger score was observed in Group 1 students as compared to Group 2 students. Significant change was noted in personality trait from Type-A towards Type-B in both groups at stressor 1 and stressor 2. Both types of intervention i.e. yoga and physical workout were found beneficial for the subjects to minimize the effect of examination stress on psycho-physiological variables however effects of yoga practice were seen quickly and were more pronounced.