The purpose of the study was to assess the impact of agility ladder training on coordination and speed endurance among football players. To achieve the purpose of the study, thirty college football players from Bengaluru city university Bengaluru Karnataka were selected as subjects. They have participated in the intercollegiate tournaments for their respective, intercollegiate football tournaments. Their age ranged from 18 to 25 years. The thirty subjects were divided into two groups of fifteen (15) subjects each. Group I underwent Agility Ladder Training (ALT), group II acted as control they did not participating any activities. Experimental group have training programmes for 12 weeks consisting of 60 morning sessions in addition to their regular programmes in their curriculum design. For that purpose, the coordination and speed endurance selected as dependent variables for this study. Coordination and speed endurance was assessed by juggling test and 150 mts run test. Since no effort was taken to pair the groups in any way and therefore to eliminate the variation in pre-test mean. Analysis of Covariance (ANCOVA) was applied as statistical tool for the present study. Significant at 0.05 level of significance. The data were examined by applying SPSS measurable package in the computer. The results of the study shows that Agility Ladder Training group have improved their coordination and speed endurance compared with control group.