The study was designed to investigate the Impact of circuit training on motor ability among inter-collegiate women football players. To investigate the study, thirty male tennis players were randomly selected from Bharathiar University, Coimbatore and their age were ranged between 21 and 24 years. The subjects were randomly assigned to two equal groups (n=15). All the subjects were divided in to two groups with 15 subjects each as experimental and control group. Group-I underwent circuit training for a period of 12 weeks and group-II acted as control who did not participate in any special training other than the regular routine. The motor ability variables such as speed and agility were selected as dependent variables. Pre and post-test random group design was used for this study. The dependent ‘t’ test was applied to determine the difference between the means of two groups. To find out whether there was any significant difference between the experimental and control groups. To test the level of significant of difference between the means 0.05 level of confidence was fixed. The result of the study shows that, there was a significant improvement takes place on speed and agility inter-collegiate women football players due to the impact of 12 weeks of circuit training and also concluded that, there was a significant difference exists between experimental and control groups in speed and agility. The control group did not improve the selected criterion variables.